GIP_HUMAN[22–51] is a bioactive peptide identified in plasma






Measured levels of Human blood levels of GIP_HUMAN[22-51],prepro using Phoenix’s GIP_HUMAN[22-51],prepro (Cat. # EK-027-48) from in house samples of human plasma and serum (N=3 for each plasma and serum)

Abstract: We recently established a new plasma peptidomic technique and comprehensively identified a large number of low-molecular weight and low-abundance native peptides using a single drop of human plasma. To discover a novel polypeptide that potently modulates the cardiovascular system, we performed a bioinformatics analysis of the large-scale identification results, sequentially synthesized the selected peptide sequences, tested their biological activities, and identified a 30-amino-acid proatherogenic peptide, GIP_HUMAN[22-51], as a potent proatherosclerotic peptide hormone. GIP_HUMAN[22-51] has a common precursor with the glucose-dependent insulinotropic polypeptide (GIP) and is located immediately N-terminal to GIP. Chronic infusion of GIP_HUMAN[22-51] into ApoE-/- mice accelerated the development of aortic atherosclerotic lesions, which were inhibited by co-infusions with an anti-GIP_HUMAN[22-51] antibody. GIP_HUMAN[22-51] increased the serum concentrations of many inflammatory and proatherogenic proteins, whereas neutralising antibodies reduced their levels. GIP_HUMAN[22-51] induced IκB-α degradation and nuclear translocation of NF-κB in human vascular endothelial cells and macrophages. Immunoreactive GIP_HUMAN[22-51] was detected in human tissues but there was no colocalization with the GIP. The plasma GIP_HUMAN[22-51] concentration in healthy humans determined using a stable-isotope tagged peptide was approximately 0.6 nM. This study discovered a novel endogenous proatherogenic peptide by using a human plasma native peptidomic resource.


Masaki T, Kodera Y, Terasaki M, Fujimoto K, Hirano T, Shichiri M. GIP_HUMAN[22–51] is a new proatherogenic peptide identified by native plasma peptidomics. Sci Rep. 2021;11(1):14470.

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027-10 [D-Ala2]-GIP (Human) 100 µg $192
EK-027-48 GIP (Human) (22-51), prepro - EIA Kit 96 wells $543
027-02 GIP (Human) 100 µg $116
027-48 GIP (Human) (22-51), prepro 200 µg $230
027-27 GIP (Mouse) 100 µg $159
EK-027-02 GIP (Human) - EIA Kit 96 wells $543
G-027-02 GIP (Human) - Purified IgG Antibody 400 µg $394
027-29 [Pro3]-GIP (Rat) 100 µg $192
027-49 [Pro3]-GIP (Mouse) 100 µg $192
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