- Protocol:
|
View/Download (PDF)
- for reference only
|
- Sample Preparation:
| Suggested Procedure |
- Measured Sample Levels:
Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results. | Human Plasma (extracted): 13.5 pg/ml
|
- Sample Extraction:
| Recommended |
- Sensitivity:
Data may differ slightly based on lot. | 13.3 pg/ml |
- Linear Range:
Data may differ slightly based on lot. | 13.3 - 301 pg/ml |
- Cross Reactivity:
Peptide |
% |
VIP (Human, Rat, Mouse, Porcine, Ovine) |
100 |
VIP (Guinea Pig) |
100 |
VIP (10-28) (Human, Porcine, Rat) |
34.6 |
VIP (Chicken) |
28 |
PACAP-27-NH2 (Human, Rat, Ovine) |
<0.02 |
VIP (1-12) (Human, Porcine, Rat) |
0 |
PHM-27 (Human) |
0 |
Substance P |
0 |
Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine) |
0 |
Secretin (Porcine) |
0 |
Glucagon (Human) |
0 |
Galanin (Rat) |
0 |
Somatostatin |
0 |
PACAP-38 (Human, Rat, Ovine) |
0 |
|
- Intra-assay variation:
| <10% |
- Inter-assay variation:
| <15% |
- Absorption:
| 325 nm |
- Max Emission:
|
420 nm |
Standard Curve:
Preliminary Plasma Levels
Sample Type |
Human Extracted* Plasma (Pooled) |
Linear Range ** |
33.2-434 pg/ml |
ED50 |
96.03 pg/ml |
Sample Level |
13.5 pg/ml |
* Extraction was performed on human plasma as described in the extraction protocol.** Linear Range varies by lot. Note: Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.
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