- Protocol:
|
View/Download (PDF)
- for reference only
|
- Sample Preparation:
| Suggested Procedure |
- Measured Sample Levels:
 Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results. | Human Plasma (extracted): 3.6 pg/ml
|
- Sample Extraction:
| Recommended |
- Sensitivity:
Data may differ slightly based on lot. | 22.6 pg/ml |
- Linear Range:
Data may differ slightly based on lot. | 22.6 - 480 pg/ml |
- Cross Reactivity:
| Peptide |
% |
| Growth Hormone Releasing Factor (Human) |
100 |
| Growth Hormone Releasing Factor (Porcine) |
100 |
| Growth Hormone Releasing Factor (Bovine) |
100 |
| Growth Hormone Releasing Factor (Ovine) |
100 |
| Growth Hormone Releasing Factor (Rat) |
0 |
| Growth Hormone Releasing Factor (1-40)(Human) |
0 |
| Growth Hormone Releasing Factor (1-37) (Human) |
0 |
| VIP (Human, Porcine, Rat) |
0 |
| Growth Hormone (Human) |
0 |
| Secretin (Human) |
0 |
|
- Intra-assay variation:
| <10% |
- Inter-assay variation:
| <15% |
- Absorption:
| 325 nm |
- Max Emission:
|
420 nm |
Standard Curve: 
Preliminary Plasma Levels
| Sample Type |
Human Extracted*Plasma (Pooled) |
| Linear Range** |
25.5-824 pg/ml |
| ED50 |
85.75 pg/ml |
| Sample Level |
3.6 pg/ml |
*
**Note:Sample level represents preliminary findings based on one experiment, using a relatively small sample size. Value is intended to demonstrate efficacy, but does not represent levels in all possible samples.
Social Network Confirmation