Ghrelin (Human) - EIA Kit, extraction-free


Catalog #
EK-031-30 96 wells $481

  • Protocol:
  • View/Download (PDF) - for reference only
  • Sample Preparation:
  • Suggested Procedure
  • Measured Sample Levels: polaroid
    Measured values represent approximate 'normal' levels in sample, but do not signify the only possible sample levels. Various factors (e.g. sample storage, assay procedure) can affect results.
  • Human Plasma: 1.12 ng/ml
    Human Serum: 3.52 ng/ml
  • Sample Extraction:
  • Not required for plasma and serum
  • Sensitivity: polaroid
    Data may differ slightly based on lot.
  • 0.13 ng/ml
  • Linear Range: polaroid
    Data may differ slightly based on lot.
  • 0.13 - 1.34 ng/ml
  • Cross Reactivity:
  • Peptide %
    Ghrelin (Human) 100
    Ghrelin (Rat) 100
    Ghrelin 1-27 (O-n-Octanoyl-Ser3) (Human) 100
    Ghrelin 1-27 (O-n-Decanoyl-Ser3) (Human) 100
    Ghrelin (Des-Octanoyl-Ser3) (Human) 100
    Ghrelin (Des-Octanoyl-Ser3) (Rat) 100
    C-Terminal of Ghrelin 100
    Ghrelin, Gln28, (Human) 100
    Ghrelin (Monkey) 85
    Ghrelin (Porcine) 0
    Secretin (Human) 0
    VIP (Human, Porcine, Rat) 0
    Prolactin-Releasing Peptide-31 (Rat) 0
    Prolactin-Releasing Peptide-31 (Human) 0
    Galanin (Rat) 0
    Galanin (Human) 0
    GH-RF (Rat) 0
    GH-RF (Human) 0
    NPY (Human) 0
    Orexin A (Human, Bovine, Mouse, Rat) 0
    Orexin B (Human) 0

  • Intra-assay variation:
  • <10%
  • Inter-assay variation:
  • <15%
  • MSDS:
  • View/Download(PDF)
Standard Curve:

The acute salivary ghrelin response to a psychosocial stress is enhanced in symptomatic patients with bulimia nervosa: a pilot study.
Monteleone P, Tortorella A, Scognamiglio P, Serino I, Monteleone AM, Maj M. Neuropsychobiology. 2012;66(4):230-6.

Plasma ghrelin level and plasma ghrelin/obestatin ratio are related to intestinal metaplasia in elderly patients with functional dyspepsia.
Kim SH, Kim JW, Byun J, Jeong JB, Kim BG, Lee KL. PLoS ONE. 2017;12(4):e0175231.

Association between maternal ghrelin levels and hyperemesis gravidarum.
Kaygusuz I, Gumus II, Yildirim M, et al. Turk J Med Sci. 2013;43:790-4.

Low to moderate dose anthracycline-based chemotherapy is associated with early noninvasive imaging evidence of subclinical cardiovascular disease.
Drafts BC, Twomley KM, D'agostino R, et al. JACC Cardiovasc Imaging. 2013;6(8):877-85.

Adjustment of gut hormones release pattern following a fixed mealtime change in human.
Kim HH, Jeon TY, Kim YJ, et al. Clin Chim Acta. 2012;414:101-4.

The role of a pre-load beverage on gastric volume and food intake: comparison between non-caloric carbonated and non-carbonated beverage.
Cuomo R, Savarese MF, Sarnelli G, et al. Nutr J. 2011;10:114.

Plasma levels of neuropeptides and metabolic hormones, and sleepiness in obstructive sleep apnea.
Sánchez-de-la-torre M, Barceló A, Piérola J, et al. Respir Med. 2011;105(12):1954-60.

Effect of ginger on gastric motility and symptoms of functional dyspepsia. World J Gastroenterol.
Hu ML, Rayner CK, Wu KL, et al. 2011;17(1):105-10.

Ghrelin modulates sympathetic nervous system activity and stress response in lean and overweight men.
Lambert E, Lambert G, Ika-sari C, et al. Hypertension. 2011;58(1):43-50.

The orexigenic peptides in children with celiac disease.
Tomasik PJ, Grzenda-Adamek Z, Sztefko K, Fyderek K. The Journal of Applied Research. 2009;9(3).

Reduction of T cell-derived ghrelin enhances proinflammatory cytokine expression: implications for age-associated increases in inflammation.
Dixit VD, Yang H, Cooper-jenkins A, Giri BB, Patel K, Taub DD. Blood. 2009;113(21):5202-5.

Plasma obestatin, ghrelin, and ghrelin/obestatin ratio are increased in underweight patients with anorexia nervosa but not in symptomatic patients with bulimia nervosa.
Monteleone P, Serritella C, Martiadis V, Scognamiglio P, Maj M. J Clin Endocrinol Metab. 2008;93(11):4418-21.

Circulating ghrelin, leptin, and soluble leptin receptor concentrations and cardiometabolic risk factors in a community-based sample.
Ingelsson E, Larson MG, Yin X, et al. J Clin Endocrinol Metab. 2008;93(8):3149-57.

Enterohormonal changes after digestive adaptation: five-year results of a surgical proposal to treat obesity and associated diseases.
Santoro S, Milleo FQ, Malzoni CE, et al. Obes Surg. 2008;18(1):17-26.

Diagnostic value of serum ghrelin levels in prostate cancer.
Mungan NA, Eminferzane S, Mungan AG, et al. Urol Int. 2008;80(3):245-8.

Clinical significance of ghrelin concentration of plasma and tumor tissue in patients with gastric cancer.
An JY, Choi MG, Noh JH, Sohn TS, Jin DK, Kim S. J Surg Res. 2007;143(2):344-9.

The role of ghrelin in weight gain and growth in epileptic children using valproate.
Gungor S, Yücel G, Akinci A, Tabel Y, Ozerol IH, Yologlu S. J Child Neurol. 2007;22(12):1384-8.

Plasma orexin and ghrelin response to the oral glucose tolerance test in obese women.
Olszanecka-glinianowicz M, Zahorska-markiewicz B, Koce?ak P, Janowska J, Semik-grabarczyk E. Endokrynol Pol. 2007;58(5):422-5.

Ghrelin and leptin levels in patients with obsessive-compulsive disorder.
Emül HM, Serteser M, Kurt E, Ozbulut O, Guler O, Gecici O. Prog Neuropsychopharmacol Biol Psychiatry. 2007;31(6):1270-4.

Peptide YY, cholecystokinin, insulin and ghrelin response to meal did not change, but mean serum levels of insulin is reduced in children with Prader-Willi syndrome.
Paik KH, Jin DK, Lee KH, et al. J Korean Med Sci. 2007;22(3):436-41.

Effects of solid versus liquid meal-replacement products of similar energy content on hunger, satiety, and appetite-regulating hormones in older adults.
Tieken SM, Leidy HJ, Stull AJ, Mattes RD, Schuster RA, Campbell WW. Horm Metab Res. 2007;39(5):389-94.

Effects of acute and chronic protein intake on metabolism, appetite, and ghrelin during weight loss.
Leidy HJ, Mattes RD, Campbell WW. Obesity (Silver Spring). 2007;15(5):1215-25.

A single session of treadmill running has no effect on plasma total ghrelin concentrations.
Burns SF, Broom DR, Miyashita M, Mundy C, Stensel DJ. J Sports Sci. 2007;25(6):635-42.

Alternate day calorie restriction improves clinical findings and reduces markers of oxidative stress and inflammation in overweight adults with moderate asthma.
Johnson JB, Summer W, Cutler RG, et al. Free Radic Biol Med. 2007;42(5):665-74.

Circulating ghrelin levels in obese women: a possible association with hypertension.
Oner-iyido?an Y, Koçak H, Gürdöl F, Oner P, Issever H, Esin D. Scand J Clin Lab Invest. 2007;67(5):568-76.

Serum ghrelin, leptin and adiponectin levels before and after weight loss: comparison of three methods of treatment--a prospective study.
Kotidis EV, Koliakos GG, Baltzopoulos VG, Ioannidis KN, Yovos JG, Papavramidis ST. Obes Surg. 2006;16(11):1425-32.

Ghrelin and the growth hormone secretagogue receptor constitute a novel autocrine pathway in astrocytoma motility.
Dixit VD, Weeraratna AT, Yang H, et al. J Biol Chem. 2006;281(24):16681-90.

Unbalanced serum leptin and ghrelin dynamics prolong postprandial satiety and inhibit hunger in healthy elderly: another reason for the "anorexia of aging".
Di francesco V, Zamboni M, Zoico E, et al. Am J Clin Nutr. 2006;83(5):1149-52.

Smoking acutely increases plasma ghrelin concentrations.
Bouros D, Tzouvelekis A, Anevlavis S, et al. Clin Chem. 2006;52(4):777-8.

Ghrelin and unacylated ghrelin stimulate human osteoblast growth via mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase (PI3K) pathways in the absence of GHS-R1a.
Delhanty PJ, Van der eerden BC, Van der velde M, et al. J Endocrinol. 2006;188(1):37-47.

Serum adipokine and ghrelin levels in nonalcoholic steatohepatitis.
Yalniz M, Bahcecioglu IH, Ataseven H, et al. Mediators Inflamm. 2006;2006(6):34295.

The levels of ghrelin, leptin, TNF-alpha, and IL-6 in liver cirrhosis and hepatocellular carcinoma due to HBV and HDV infection.
Ataseven H, Bahcecioglu IH, Kuzu N, et al. Mediators Inflamm. 2006;2006(4):78380.

Increased density of ghrelin-expressing cells in the gastric fundus and body in Prader-Willi syndrome.
Choe YH, Song SY, Paik KH, et al. J Clin Endocrinol Metab. 2005;90(9):5441-5.

Ghrelin in preterm and term newborns: relation to anthropometry, leptin and insulin.
Ng PC, Lee CH, Lam CW, Chan IH, Wong E, Fok TF. Clin Endocrinol (Oxf). 2005;63(2):217-22.

Comparative study of plasma ghrelin levels in women with polycystic ovary syndrome, in hyperandrogenic women and in normal controls.
Panidis D, Farmakiotis D, Koliakos G, et al. Hum Reprod. 2005;20(8):2127-32.

Leptin and ghrelin levels in patients with obstructive sleep apnea syndrome.
Ulukavak ciftci T, Kokturk O, Bukan N, Bilgihan A. Respiration. 2005;72(4):395-401.

Circulating ghrelin in patients with chronic obstructive pulmonary disease.
Luo FM, Liu XJ, Li SQ, Wang ZL, Liu CT, Yuan YM. Nutrition. 2005;21(7-8):793-8.

Hyperghrelinemia does not accelerate gastric emptying in Prader-Willi syndrome patients.
Choe YH, Jin DK, Kim SE, et al. J Clin Endocrinol Metab. 2005;90(6):3367-70.

Plasma ghrelin and resistin concentrations are suppressed in infants of insulin-dependent diabetic mothers.
Ng PC, Lee CH, Lam CW, Wong E, Chan IH, Fok TF. J Clin Endocrinol Metab. 2004;89(11):5563-8.

No changes in serum ghrelin levels in female patients with bulimia nervosa.
Nakazato M, Hashimoto K, Shiina A, et al. Prog Neuropsychopharmacol Biol Psychiatry. 2004;28(7):1181-4.

Correlation between fasting plasma ghrelin levels and age, body mass index (BMI), BMI percentiles, and 24-hour plasma ghrelin profiles in Prader-Willi syndrome.
Paik KH, Jin DK, Song SY, et al. J Clin Endocrinol Metab. 2004;89(8):3885-9.

Ghrelin inhibits leptin- and activation-induced proinflammatory cytokine expression by human monocytes and T cells.
Dixit VD, Schaffer EM, Pyle RS, et al. J Clin Invest. 2004;114(1):57-66.

Ghrelin, leptin and insulin levels after restrictive surgery: a 2-year follow-up study.
Nijhuis J, Van dielen FM, Buurman WA, Greve JW. Obes Surg. 2004;14(6):783-7.

Effect of weight reduction on serum ghrelin and TNFalpha concentrations in obese women.
Zahorska-markiewicz B, Mizia-stec K, Olszanecka-glinianowicz M, Janowska J. Eur J Intern Med. 2004;15(3):172-175.

Umbilical cord ghrelin concentrations in small- and appropriate-for-gestational age newborn infants: relationship to anthropometric markers.
Onal EE, Cinaz P, Atalay Y, et al. J Endocrinol. 2004;180(2):267-71.

Expression of ghrelin and biological activity of specific receptors for ghrelin and des-acyl ghrelin in human prostate neoplasms and related cell lines.
Cassoni P, Ghé C, Marrocco T, et al. Eur J Endocrinol. 2004;150(2):173-84.

Helicobacter pylori has no effect on plasma ghrelin levels.
Gokcel A, Gumurdulu Y, Kayaselcuk F, et al. Eur J Endocrinol. 2003;148(4):423-6.

Hypothalamic growth hormone secretagogue receptor regulates growth hormone secretion, feeding, and adiposity.
Shuto Y, Shibasaki T, Otagiri A, et al. J Clin Invest. 2002;109(11):1429-36.