Adrenomedullin-5 (Porcine) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry
Tissue Sample	Human Placenta Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No Primary Antibody
Pretreatment	N/A
Blocking	2% Normal Goat Serum, 3% H₂O₂
Primary Antibody	Rabbit anti-Adrenomedullin-5 (Porcine) Antiserum (Cat. No.: H-010-40)
Optimal Dilution	1:500
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 sec

Catalog #

H-010-40

Family

Adrenomedullin

Standard Size

100 µl

Sequence

His - Gln - Val - Ser - Leu - Lys - Ser - Gly - Arg - Leu - Cys - Ser - Leu - Gly - Thr - Cys - Gln - Thr - His - Arg - Leu - Pro - Glu - Ile - Ile - Tyr - Trp - Leu - Arg - Ser - Ala - Ser - Thr - Lys - Glu - Leu - Ser - Gly - Lys - Ala - Gly - Arg - Lys - Pro - Gln - Asp - Pro - Tyr - Ser - Tyr - NH2 Disulfide bridge between Cys11 & Cys16

Species

Porcine

Host

Rabbit

Immunogen

Adrenomedullin-5 (Porcine)

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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