Glucagon (19-29) (Human, Rat, Mouse, Porcine, Bovine) - Antibody for Immunohistochemistry

Image

H-028-05-2469-3-H-500 H-028-05-2470-3-H-500

Protocol for Immunohistochemistry
Tissue Sample	Human pancreas tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit anti-Glucagon (19-29) (H,R,M) antibody (Cat. No.: H-028-05)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-028-05

Standard Size

100 µl

Species

Human, Rat, Mouse, Porcine, Bovine

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

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