Nesfatin-3, C-Terminal (Human) - Antibody for Immunohistochemistry

Image

Protocol for Immunohistochemistry:
Tissue Sample	Human Brain Tissue
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	3% H₂O₂, 2% Normal Goat Serum
Primary Antibody	Rabbit anti-Nesfatin-3, C-Terminal (Human) antibody (Cat. No.: H-003-27)
Optimal Dilution	1:500
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-003-27

Standard Size

100 µl

Sequence

Leu - Gln - Gln - Gly - Ile - Pro - Pro - Ser - Gly - Pro - Ala - Gly - Glu - Leu - Lys - Phe - Glu - Pro - His - Ile

Species

Human

Host

Rabbit

Reconstitution

Reconstitute with 100 ul distilled water.

Storage Condition

For optimal results, use the antibody as soon as possible after reconstitution. Store in lyophilized form unless needed and reconstitute immediately before use. Once reconstituted, the antibody should be stable for a few days at -4°C. For storage up to a few months, prepare small aliquots after reconstitution and freeze at -20°C or -80°C. Repeated freeze thaw cycles should be strictly avoided.

Recommended Dilution Factor

Indirect ELISA: 1:3200

Cross Reactivity

*Peptide*	*% cross reactivity*
NUCB 2 (401-420) (Human)	100

Alphabet_N_