BAFF-R [Cys0] (151-175) (Mouse) / BAFF-R [Cys0] (159-183) (Human)

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Protocol for Immunohistochemistry:
Tissue Sample	Rat Spleen
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	intact
Blocking	2% Normal Goat Serum
Primary Antibody	BAFF-R (159-183) (Human)/BAFF-R (151-175), Cys⁰, Antibody (Catalog No.: H-030-51)
Optimal Dilution	1:100
Secondary Antibody	Goat Anti-Rabbit IgG, Biotinylated (1:400)
Amplification	ABC (Vector)
Detection System	HRP
Substrate	DAB (Sigma)
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-030-51

Standard Size

50 µl

Species

Human, Mouse

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

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